Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the inclusion in this Amendment No. 14 to the Registration Statement of Intensity Therapeutics, Inc. on Form S-1 (No. 333-260565) to be filed on or about June 9, 2023 of our report dated March 21, 2023, except for the 2^nd paragraph of Note A[1], and its related effects to the financial statements, which is as of May 18, 2023, on our audits of the financial statements as of December 31, 2022 and 2021 and for each of the years then ended. Our report includes an explanatory paragraph about the existence of substantial doubt concerning the Company’s ability to continue as a going concern. We also consent to the reference to our firm under the caption “Experts” in this Registration Statement.

/s/ EisnerAmper LLP

EISNERAMPER LLP

New York, New York

June 9, 2023