Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in the Registration Statements of Intensity Therapeutics, Inc. on Forms S-3 (Nos. 333-280681 and 333-280684), Forms S-8 (Nos. 333-274141, 333-280686, 333-288379, and 333-292923), and Forms S-1 (No. 333-283797 and 333-286683) of our report dated March 27, 2026, on our audits of the financial statements as of December 31, 2025 and 2024 and for each of the years then ended, which report is included in this Annual Report on Form 10-K to be filed on or about March 27, 2026. Our report includes an explanatory paragraph about the existence of substantial doubt concerning the Company's ability to continue as a going concern.

/s/ EisnerAmper LLP

EISNERAMPER LLP
New York, New York
March 27, 2026